Strange encounter in the red channel/RPi HQ Camera

No. At least in the original version, there is no ALSC section at all. Haven’t checked lately, but this section should not be present. That was one of the important modifications of the scientific tuning file.

Your problem might be caused by the illumination itself. Have a walk through the forums for various solutions without a 3D-printer, like integrating spheres out of kitchen stuff or various type of plastic diffusors. Check how even your illumination is with another camera if possible.

The uneveness could also be caused by the lens you are using. Or your setup. Some kind of illumination requires an appropriate field lens for proper operation.

For various reasons, I recommend a diffuse lightsource. Best approach here are integrating sphere setups. Or very good diffusors. Sometimes it helps to use several sheets stacked together. Such a setup kills brightness, so be sure to work with a sufficiently bright illumination source.

And please: your request has nothing to do with the original topic. Open a new topic. In that way other users are also able to search for and find things easier. Concerning your original question: a photo of your basic setup and of the results you are getting would help others to comment better to your question.

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